Which chamber does the pulmonary valve lead from?

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The pulmonary valve is the outflow valve of the right ventricle. It opens when the right ventricle contracts to push blood into the pulmonary trunk and then into the lungs. It closes to prevent backflow into the ventricle during relaxation. The other chambers aren’t directly associated with the pulmonary valve: the right atrium feeds the right ventricle through the tricuspid valve, the left atrium feeds the left ventricle through the mitral valve, and the left ventricle passes blood to the aorta via the aortic valve. So, the chamber the pulmonary valve leads from is the right ventricle.